Conclusions: Intra-species variation was observed among the isolates from this region of Polymorphism genotypes were sub-typed into seven sequence types including three novel Sequence types (STs) from The 2 allele numbers for CAP59, SOD1,Īnd URA5, 3 for LAC1 and PLB1 and 4 for GPD1 and IGS1 per gene loci were noted. Var grubii isolates were divided into two genotypes VNI (%) and VNII (%). Results: The phylogenetic tree was made to determine the relatedness between the isolates. AllĢ8 DNA sequences were deposited in Genbank under the following accession numbers MF580727 to MF580754. Subjected to genotyping by Multilocus sequence typing (MLST) method using 8 different house-keeping loci. Methods: Out of 47 clinical cryptococcal isolates from suspected cryptococcosis 26 were further About 26 of these isolates were molecular typed to identify the prevalent molecular types from Western neoformans from cryptococcosis patents during Apr 2010 to Jun 2015 from different neoformans has a two-fold higher risk of fatality in cryptococcosis coinfected with HIV patients. Introduction: Cryptococcosis is a fungal infection predominantly caused by Cryptococcus neoformans. In this patient, India ink result was negative, urease test positivity within. Of the other two patients, one was a farmer with disseminated tuberculosis and other was a 42 year old male shopkeeper with no known risk factors. and hemiparesis due to a cerebrovascular accident. Details of genotyping of these isolates have been published in a study from India by Chowdhary et al. neoformans var grubii, molecular type VNI. Mating type PCR results showed that all isolates were mating type α (MAT α) and PCR fingerprinting analyses identified all forty six strains as C. All the isolates were susceptible to amphotericin B, fluconazole, ketoconazole, voriconazole and itraconazole, except one (2.1%) which was resistant to 5- fluorocytosine. All strains were negative in canavanine-glycine-bromthymol blue (CGB) test, hence identified as Cryptococcus neoformans var grubii. Cryptococcus neoformans grew in routine fungal culture media, most often within two days of incubation, brown pigmented colonies appeared on niger seed agar within four days at 30☌ and urease test was positive. budding yeast cells ( Figure 1) were seen in forty five specimens on India ink preparation (98%).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |